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KMID : 0364820120480040293
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.293 ~ p.297
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Characterization of a Chitinase Gene and Screening of Cold Active Chitinase from Polar Microorganisms
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Park Yu-Kyung
Kim Jung-Eun
Lee Hyoung-Seok
Kim Ji-Hyun
Park Ha-Ju
Kim Doc-Kyu
Park Mi-Ra
Yim Joung-Han
Kim Il-Chan
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Abstract
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Of the 169 strains of microorganisms stored in Polar and Alpine Microbial Collection of Korea Polar Research
Institute, 27 strains were selected for their chitinase activity on ZoBell plates supplemented with 0.4% colloidal chitin.
Among them, PAMC 21693 strain have shown the highest chitinolytic enzyme activity toward pNP-(GlcNAc)1 at low
temperature and the highest growth rate at 4℃. We cloned a full-length chitinase gene of 2,857 bp which contains an
open reading frame of 2,169 bp encoding 872-amino acid polypeptide. Recombinant chitinase protein was expressed
in E. coli and its molecular weight was confirmed 96 kDa. In this paper, we suggest the potential use of cold-active
chitinase from polar microorganisms in the field of biotechnology.
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KEYWORD
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chitinase, cold-active chitinase, iPCR, polar microorganisms, screening
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